377 Exploring neutrophil nuclear dynamics and neutrophil extracellular traps using expansion microscopy

Neutrophils are critical to the inflammatory process and tissue homeostasis. The lobulated neutrophil nucleus has a unique membrane composition, yielding deformable structure that is highly responsive environment allows for efficient migration site of inflammation. Furthermore, neutrophils possess an arsenal antimicrobial defense mechanisms, including expulsion extracellular traps (NETs), composed nuclear DNA studded with cellular proteins. However, dysregulated or excessive activation implicated in many malignant diseases, highlighting need understand biology. To date, thorough study morphology especially been greatly limited by resolution limits inherent microscopy thus requires expensive specialized super-resolution equipment. Therefore, we have implemented expansion imaging neutrophils, as it cost- time-effective can easily be performed using conventional We used this novel technique characterize distribution chromatin, histone H1, nucleophosmin (NPM1) both unstimulated those undergoing NETosis. also labeled lamins B1 B2 envelope markers myeloperoxidase (MPO) cytoplasmic protein. gain was calculated assessing colocalization H1 chromatin Pearson’s coefficient. Line scan analysis NPM1 throughout showed increase detail. In conclusion, tool dynamics image proteins at microscopes. This method adapted other (immune) cell types valuable analyze morphology.